Mucking about in FlowJo is fun, especially when you can get immediate visual feedback from changing a transformation setting. For the below, I've chosen to plot only in Pseudocolor and vary only one setting at a time in FlowJo Mac version 9.3.1. For a series of lovely movies that manipulate all three settings at once, check out the Daily Dongle's Width Basis Titration.
In short, reducing the width basis value compresses the linear space around zero. See here for settings at -1, -10, -100, and -1000. It results in more or less plotspace devoted to the Negative and Dim populations. I find that customizing this setting to be most useful for investigating dim events.
For the positive decades, the setting reflects the first decade that is displayed after the linear space ends. It feels like the plot zooms-in on the intermediately bright populations. See here for settings as applied at 3, 3.5, 4, 4.5, and 5.
When it comes to the additional negative decades, settings result in more and more space devoted in plotspace for negative events. See here for 0, 0.5, 1, 1.5. I don't find altering them very useful.
I'd like to be able to do this with my FSC and SSC axes. I'm using the accuri C6 cytometer and when I import into FlowJo 9.3.1, everything is all bunch up in the lower lefthand corner. Any suggestions?
Basically, I'm looking for this kind of setting, but in the 9.3.1: http://www.flowjo.com/v762/en/prefsfcs.html
Posted by: Ben C. | May 12, 2011 at 07:03 PM
Hi Ben, sorry to be sooo late in responding. Use the derived parameters function on the Mac side to get your plots to look like they were on the C6.
Posted by: Suzanne | July 19, 2011 at 09:11 PM