Using FlowJo to calculate compensation

Compensation becomes a thorny issue for CellQuest users who wish to work with GFP and other colors. A quick work-around is to first collect Calibur data uncompensated. Then, load your FCS files into FlowJo and define your compensation matrix based on your single-stain histograms. Download this PowerPoint tutorial tutorial for more details.

Comments

[Noah]

Nice tutorial for defining compensation matrix in FlowJo. You may want to tell people that this is what they really should do, instead of offering it as a workaround for GFP. Online compensation (collecting compensated data) *permanently* alters your data - you can never get your raw data back.

[maciej simm]

I really like this. Can I link it to my bulletin board and blog for other folks to see?

http://flowjo.typepad.com/
http://mail.treestar.com/phpbb2/

Maciej Simm
tech support

[Suzanne Mertens]

Go ahead Maciej - link it up.

Noah, you're not the only one with that bright idea. Hit PubMed and check out what the Herzenberg lab has recently published.