In a collaborative effort with FlowJo, Steve Perfetto, and Ryan Brinkman's group, I'm collecting statistics from manual gating on bead files. The pupose is to define a reference range of statistics that adequately describes the variation from one manual analyst to another. The files in the analysis set are completely artificial, and eliminate any experience bias that may occur if real-world samples were provided to a variety of individuals possessing different levels of exposure to real population gating. The context for this collection is development of an evaluation method for automated gating algorithm. More on automated cluster detection and the related Q and B platform in development can be found on the FlowJo and FlowDx websites.
Members of the Brinkman lab are well-known in the open-source community of Bioconductor/R for their flow cytometry packages, and the exercise is expected to have significant benefit to developers on any platform. Would you like to get involved in cluster development too? Or you can download the files and instructions here. Send an email to my FlowJo account: suzanne at treestar dot com and I can walk you through the analysis. The gist of it is in the pic above.
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