I found that using a positive control for douplets helps figure out the best gating method for doublet discrimination. Use this Doublet Discrimination on the FACSAria PDF to see plotting of Area, Height and Width for chicken erythrocyte nuclei from BD's DNA QC Particle Kit. I'd be happy to hear your comments.
With thanks to the Emory Vaccine Center's flow cytometry core and Dr. John Altman.
great man :D
Posted by: bedroom furniture | July 28, 2013 at 12:23 PM