CD117... aka C-Kit... cytokine receptor for stem cell factor (SCF).
According to Canonico B, Felici C, Papa S.... c-kit expression in acute leukemia can be considered as an indicator of myeloid origin of tumor cells as it is rarely expressed in lymphoid blast cells. Ortolani in Flow Cytometry of Hematological Malignancies describes CD117 staining as "less likely" in AML-M4, "can be expressed in" more immature AML-5a, and "often CD117-" in more mature AML-5a. In AML-5b, Dunphy/Orton/Mantel show flow data with dim CD117 expression. This article had a nice series of plots for CD117 vs CD64, excerpts of which are below with ommision of other panel components:
| healthy normal | CD117- / CD64+ | monos |
| CMML | CD117- / CD64+ | "25% mature monos" |
| CML-2 | CD117 very dim / CD64+ | "2:3 ratio immature to mature" |
| CML-1 | CD117 dim / CDE64- | "predominantly mature monos" |
| AML-5b | CD117 int / CD64+ | "mostly immature monocytic" |
From the BD TDS for #550412... clone YB5.B8... listed in the references is Ashman et al. describing epitope maping and function studies, in a 1994 article in J Cell Physiol. Mast cell tumors can be diagnosed via CD117 staining, according to Leong et al. in the second edition of "Manual of Diagnostic Cytology". Ashman's review of the biology of c-Kit is fine but doesn't address the issue of a biological basis for transient incidents of high background staining by flow.
Comments