Buffers

It should come as no surprise that the buffers used in sorting - ie sample buffer, and collection buffer -  are quite different from one another AND from culture media.

     Scripps Sort Buffer... HEPES/EDTA/DNAse, polypropylene vs polystyrene

     ThermoFisher... Glutamax vs Glutamine

     UNC Flow Core... trypsin vs soybean trypsin